2. Academic Validation
  3. RNASeq profiling of retinal pigment epithelial cells derived from induced pluripotent stem cells revealed 3 genes involved in lipid homeostasis in age-related macular degeneration

RNASeq profiling of retinal pigment epithelial cells derived from induced pluripotent stem cells revealed 3 genes involved in lipid homeostasis in age-related macular degeneration

  • Exp Eye Res. 2024 Jul 10:246:109999. doi: 10.1016/j.exer.2024.109999.
Audrey Voisin 1,  Amaury Pénaguin 2,  Afsaneh Gaillard 3,  Nicolas Leveziel 4
Affiliations

Affiliations

  • 1 University of Poitiers, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86073, France; INSERM, U1084, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86022, France; CHU Poitiers, Poitiers, F-86021, France. Electronic address: audrey.voisin@univ-poitiers.fr.
  • 2 University of Poitiers, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86073, France; INSERM, U1084, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86022, France; Laboratoires THEA, Clermont-Ferrand, France.
  • 3 University of Poitiers, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86073, France; INSERM, U1084, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86022, France.
  • 4 University of Poitiers, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86073, France; INSERM, U1084, Laboratoire de Neurosciences Expérimentales et Cliniques, Equipe Thérapie cellulaire dans les pathologies cérébrales, Poitiers, F-86022, France; CHU Poitiers, Poitiers, F-86021, France.
PMID: 38996902 DOI: 10.1016/j.exer.2024.109999
Abstract

Age-related macular degeneration (AMD) is characterized by visual impairment observed in elderly population. Two forms of the disease are generally described, the atrophic (AMDa) and exudative forms (AMDe). Up until now, no curative treatment is available for this disease. The retinal pigment epithelium (RPE) plays a central role in the pathogenesis of age-related macular degeneration. Here, involvement of RPE dysfunction in AMD onset and progression was analyzed by a comparison of transcriptome profiles of hiPSC-RPE derived from healthy individuals or individuals affected by AMDa or AMDe. The analysis highlighted almost 1000 genes differentially expressed between the three comparison groups. Among these genes, 33 genes were already known to be involved in AMD pathogenesis. To establish an AMD genetic signature, we focused on genes differentially expressed in both AMDa/e cell lines compared to control cells and focused on the three genes (ABCA1, RPN2, RB1CC1) that were related to lipidic homeostasis. Differences in level expression of these three genes are found not only in control and AMDa/e cell lines, but also between AMDa and AMDe populations.

Keywords

Cell signaling; Cholesterol; Genomics; Lipids; Retina.

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