2. Academic Validation
  3. Mutual regulation between histone methyltransferase Suv39h1 and the Wnt/β-catenin signaling pathway promoted cell proliferation and inhibited apoptosis in bone marrow mesenchymal stem cells exposed to hydroquinone

Mutual regulation between histone methyltransferase Suv39h1 and the Wnt/β-catenin signaling pathway promoted cell proliferation and inhibited apoptosis in bone marrow mesenchymal stem cells exposed to hydroquinone

  • Toxicology. 2024 Nov:508:153932. doi: 10.1016/j.tox.2024.153932.
Tao Xu 1,  Yilin Shen 2,  Runmin Guo 3,  Chiheng Luo 4,  Yibo Niu 5,  Zhilong Luo 6,  Zhongxin Zhu 7,  Zehui Wu 8,  Xinyu Zhao 9,  Hao Luo 10,  Yuting Gao 11
Affiliations

Affiliations

  • 1 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: 15955716703@163.com.
  • 2 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: 13192852105@163.com.
  • 3 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: runmin.guo@gdmu.edu.cn.
  • 4 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: 1525915007@qq.com.
  • 5 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: 3613843375@qq.com.
  • 6 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: ymenend@163.com.
  • 7 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: zhuzhongxin@gdmu.edu.cn.
  • 8 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: walker9823@163.com.
  • 9 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: zhaoxy@gdmu.edu.cn.
  • 10 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: lh426@gdmu.cn.
  • 11 Shunde Women and Children's Hospital of Guangdong Medical University, School of Public Health, Key Laboratory of Environmental Medicine, Guangdong Medical University, Guangdong, China. Electronic address: gaoyuting@gdmu.edu.cn.
PMID: 39179171 DOI: 10.1016/j.tox.2024.153932
Abstract

Hydroquinone (HQ), a metabolite of benzene, is frequently utilized as a surrogate for benzene in in vitro studies and is associated with the development of acute myeloid leukemia (AML). In the hemotoxicity caused by benzene and HQ, cell Apoptosis plays a key role. However, the molecular mechanisms underlying HQ are unknown. Studies have indicated that Suv39h1 is involved in regulating cell division and proliferation by regulating histone H3K9me3. Meanwhile, the Wnt/β-catenin signaling pathway also plays a significant role in cell proliferation and Apoptosis. Therefore, this study was aimed at exploring the regulatory role of Suv39h1 and the Wnt/β-catenin signaling pathway in the effects of HQ on bone marrow mesenchymal stem cells (BMSCs), as well as its influence on cell proliferation and Apoptosis. The results demonstrated that HQ elevated the levels of Suv39h1 and H3K9me3 and activated the Wnt/β-catenin signaling pathway by upregulating β-catenin, Wnt2b, c-Myc, and Cyclin D1 and downregulating Wnt5a, resulting in an increase in cell growth and a decrease in Apoptosis. Suv39h1 knockdown inhibited the Wnt/β-catenin signaling pathway. Meanwhile, inhibition of the Wnt/β-catenin signaling pathway resulted in the down-regulation of Suv39h1 and H3K9me3 in BMSCs. They both promoted cell proliferation and inhibited Apoptosis in the effects of HQ on BMSCs by downregulating the expression of Cyt-C, Bax, Caspase 3, and Caspase 9 and upregulating the expression of Bcl-xL. Therefore, we concluded that Suv39h1 and the Wnt/β-catenin signaling pathway may mutually regulate each other in the effects of HQ on BMSCs in order to ameliorate the altered function of BMSCs.

Keywords

Apoptosis; BMSCs; HQ; Suv39h1; Wnt/β-catenin signaling pathway.

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