Nek2-IN-5

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Nek2-IN-5 (compound 6) is a potent and irreversible Nek2 ((Never in mitosis gene a)-related kinase 2) inhibitior. Nek2-IN-5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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Nek2-IN-5 Chemical Structure

CAS No. : 1507367-00-1

Size	Price	Stock	Quantity
1 mg	USD 569	Get quote 3 - 4 weeks 4 - 5 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
5 mg		Get quote
10 mg		Get quote

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•Related Small Molecules:

•Related Proteins:

View All NEKs Isoform Specific Products:

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NEK1 NEK2 NEK6 NEK7

Biological Activity
Purity & Documentation
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Description

Nek2-IN-5 (compound 6) is a potent and irreversible Nek2 ((Never in mitosis gene a)-related kinase 2) inhibitior^[1]. Nek2-IN-5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

NEK2

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	GI₅₀	18.1 μM Compound: 66	Growth inhibition against human HCT116 cells incubated for 72 hrs Growth inhibition against human HCT116 cells incubated for 72 hrs	[PMID: 33479670]
HeLa	GI₅₀	32.2 μM Compound: 66	Growth inhibition of human HeLa cells incubated for 72 hrs Growth inhibition of human HeLa cells incubated for 72 hrs	[PMID: 33479670]
HeLa	GI₅₀	47.1 μM Compound: 66	Growth inhibition of human HeLa cells measured after 92 hrs by CellTiter Blue assay Growth inhibition of human HeLa cells measured after 92 hrs by CellTiter Blue assay	[PMID: 33479670]
MCF7	GI₅₀	6.6 μM Compound: 66	Growth inhibition against human MCF-7 cells incubated for 72 hrs Growth inhibition against human MCF-7 cells incubated for 72 hrs	[PMID: 33479670]
Rec1	GI₅₀	1.6 μM Compound: 66	Growth inhibition against human Rec-1 cells incubated for 72 hrs Growth inhibition against human Rec-1 cells incubated for 72 hrs	[PMID: 33479670]
SK-BR-3	GI₅₀	2.2 μM Compound: 66	Growth inhibition against human SKBR3 cells incubated for 72 hrs Growth inhibition against human SKBR3 cells incubated for 72 hrs	[PMID: 33479670]
T47D	GI₅₀	3.6 μM Compound: 66	Growth inhibition against human T47D cells incubated for 72 hrs Growth inhibition against human T47D cells incubated for 72 hrs	[PMID: 33479670]
U2OS	GI₅₀	71 μM Compound: 66	Growth inhibition of human U2OS cells measured after 92 hrs by CellTiter Blue assay Growth inhibition of human U2OS cells measured after 92 hrs by CellTiter Blue assay	[PMID: 33479670]

Molecular Weight

292.30

Formula

C₁₅H₁₂N₆O

CAS No.

1507367-00-1

SMILES

O=C(CC1=CC=CC(NC2=NC(C#C)=C3N=CNC3=N2)=C1)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Lebraud H, et al. Model system for irreversible inhibition of Nek2: thiol addition to ethynylpurines and related substituted heterocycles. Org Biomol Chem. 2014 Jan 7;12(1):141-8. [Content Brief]

Nek2-IN-5 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nek2-IN-51507367-00-1NEKsNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed Kinases6-ethynylpurinecancercysteineInhibitorinhibitorinhibit

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