Procaine hydrochloride

Name: Procaine hydrochloride
Brand: MedChemExpress
SKU: HY-B0546A
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0546A Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Procaine hydrochloride is a DNA-demethylating agent. Procaine hydrochloride acts through multiple targets and has a slow onset and a short duration of action.

For research use only. We do not sell to patients.

Procaine hydrochloride Chemical Structure

CAS No. : 51-05-8

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500 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
1 g	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g	USD 80	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Procaine hydrochloride:

Procaine In-stock
Procaine-d₄ hydrochloride Get quote
Procaine hydrochloride (Standard) Get quote

1 Publications Citing Use of MCE Procaine hydrochloride

•Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Procaine hydrochloride is a DNA-demethylating agent. Procaine hydrochloride acts through multiple targets and has a slow onset and a short duration of action^[1]^[2].

In Vitro

Procaine hydrochloride (0.01-100 microM) inhibited the 5-HT3 receptor-mediated inward current in the whole-cell patch clamp recording. Procaine appears to produce a competitive inhibition on 5-HT3 receptors with a KD of 1.7 microM^[1]. Procaine is a DNA-demethylating agent that produces a 40% reduction in 5-methylcytosine DNA content as determined by high-performance capillary electrophoresis or total DNA enzyme digestion. Procaine can also demethylate densely hypermethylated CpG islands. Procaine also has growth-inhibitory effects in these cancer cells, causing mitotic arrest^[2]. Procaine functions as an excitant of limbic system cells, and that procaine alters synaptic transmission in some, but not all, output pathways from the amygdale^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

272.77

Formula

C₁₃H₂₁ClN₂O₂

CAS No.

51-05-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCN(CC)CC)C1=CC=C(N)C=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (183.30 mM)

DMSO : 50 mg/mL (183.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6661 mL	18.3305 mL	36.6609 mL
5 mM	0.7332 mL	3.6661 mL	7.3322 mL
10 mM	0.3666 mL	1.8330 mL	3.6661 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 130 mg/mL (476.59 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (271 KB) SDS (420 KB)

COA (244 KB) RP-HPLC (245 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Fan, P. and F.F. Weight, Procaine impairs the function of 5-HT3 receptor-ion channel complex in rat sensory ganglion neurons. Neuropharmacology, 1994. 33(12): p. 1573-9. [Content Brief]

[2]. Villar-Garea, A., et al., Procaine is a DNA-demethylating agent with growth-inhibitory effects in human cancer cells. Cancer Res, 2003. 63(16): p. 4984-9. [Content Brief]

[3]. Adamec, R.E. and C. Stark-Adamec, The effects of procaine HCl on population cellular and evoked response activity within the limbic system of the cat. Evidence for differential excitatory action of procaine in a variety of limbic circuits. Prog Neuropsychopharmacol Biol Psychiatry, 1987. 11(4): p. 345-64. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.6661 mL	18.3305 mL	36.6609 mL	91.6523 mL
	5 mM	0.7332 mL	3.6661 mL	7.3322 mL	18.3305 mL
	10 mM	0.3666 mL	1.8330 mL	3.6661 mL	9.1652 mL
	15 mM	0.2444 mL	1.2220 mL	2.4441 mL	6.1102 mL
	20 mM	0.1833 mL	0.9165 mL	1.8330 mL	4.5826 mL
	25 mM	0.1466 mL	0.7332 mL	1.4664 mL	3.6661 mL
	30 mM	0.1222 mL	0.6110 mL	1.2220 mL	3.0551 mL
	40 mM	0.0917 mL	0.4583 mL	0.9165 mL	2.2913 mL
	50 mM	0.0733 mL	0.3666 mL	0.7332 mL	1.8330 mL
	60 mM	0.0611 mL	0.3055 mL	0.6110 mL	1.5275 mL
	80 mM	0.0458 mL	0.2291 mL	0.4583 mL	1.1457 mL
	100 mM	0.0367 mL	0.1833 mL	0.3666 mL	0.9165 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.