2-Ethylbutyric acid

Name: 2-Ethylbutyric acid
Brand: MedChemExpress
SKU: HY-W004154
Rating: 4.5 (1 reviews)

Cat. No.: HY-W004154 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2-Ethylbutyric acid acts as an internal standard (IS) in a standard addition calibration method for the VFA analysis of faeces.

For research use only. We do not sell to patients.

2-Ethylbutyric acid Chemical Structure

CAS No. : 88-09-5

Size	Price	Stock	Quantity
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g	USD 70	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

2-Ethylbutyric acid acts as an internal standard (IS) in a standard addition calibration method for the VFA analysis of faeces^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Hepatocyte	EC₅₀	33.74 μM Compound: 1	Reduction of propionyl-CoA in human hepatocytes derived from propionic acidemia patient pretreated for 30 mins followed by 13C-isoleucine addition and measured after 1 hr by MS/MS analysis Reduction of propionyl-CoA in human hepatocytes derived from propionic acidemia patient pretreated for 30 mins followed by 13C-isoleucine addition and measured after 1 hr by MS/MS analysis	[PMID: 33848153]

Molecular Weight

116.16

Formula

C₆H₁₂O₂

CAS No.

88-09-5

Appearance

Liquid (Density: 0.92 g/cm³)

Color

Colorless to light yellow

SMILES

CCC(CC)C(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (860.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.6088 mL	43.0441 mL	86.0882 mL
5 mM	1.7218 mL	8.6088 mL	17.2176 mL
10 mM	0.8609 mL	4.3044 mL	8.6088 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (645 KB)

COA (248 KB) HNMR (236 KB)

Handling Instructions (2659 KB)

References

[1]. Cruwys JA, et al. Development of a static headspace gas chromatographic procedure for the routine analysis of volatile fatty acids in wastewaters. J Chromatogr A. 2002 Feb 1;945(1-2):195-209. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	8.6088 mL	43.0441 mL	86.0882 mL	215.2204 mL
	5 mM	1.7218 mL	8.6088 mL	17.2176 mL	43.0441 mL
	10 mM	0.8609 mL	4.3044 mL	8.6088 mL	21.5220 mL
	15 mM	0.5739 mL	2.8696 mL	5.7392 mL	14.3480 mL
	20 mM	0.4304 mL	2.1522 mL	4.3044 mL	10.7610 mL
	25 mM	0.3444 mL	1.7218 mL	3.4435 mL	8.6088 mL
	30 mM	0.2870 mL	1.4348 mL	2.8696 mL	7.1740 mL
	40 mM	0.2152 mL	1.0761 mL	2.1522 mL	5.3805 mL
	50 mM	0.1722 mL	0.8609 mL	1.7218 mL	4.3044 mL
	60 mM	0.1435 mL	0.7174 mL	1.4348 mL	3.5870 mL
	80 mM	0.1076 mL	0.5381 mL	1.0761 mL	2.6903 mL
	100 mM	0.0861 mL	0.4304 mL	0.8609 mL	2.1522 mL