Azoxymethane (Synonyms: AOM)

Name: Azoxymethane
Brand: MedChemExpress
SKU: HY-111375
Rating: 5.0 (11 reviews)

Cat. No.: HY-111375 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

For research use only. We do not sell to patients.

Azoxymethane Chemical Structure

CAS No. : 25843-45-2

Size	Stock	Quantity
Solvent
5 mg (135 mM * 500 μL in Water)	In-stock	Estimated Time of Arrival: December 31
Solvent
10 mg (135 mM * 1 mL in Water)	In-stock	Estimated Time of Arrival: December 31
Solvent
25 mg (135 mM * 2.5 mL in Water)	In-stock	Estimated Time of Arrival: December 31
Solvent
50 mg (135 mM * 5 mL in Water)	In-stock	Estimated Time of Arrival: December 31
Solvent
100 mg (135 mM * 10 mL in Water)	In-stock	Estimated Time of Arrival: December 31

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Based on 11 publication(s) in Google Scholar

11 Publications Citing Use of MCE Azoxymethane

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Description

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

In Vitro

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts. On an equal protein basis, hepatic microsomes are much more active than SI and colon microsomes in NADPH-dependent Azoxymethane bioactivation and N⁷-mG adduct formation. Hepatic microsomes show the highest activity in the hydroxylation of Azoxymethane, followed by SI and colon microsomes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Azoxymethane can be used to induce colorectal cancer models.

Induction of Colonic

Background

Azoxymethane induces induces G to A transitions and leads to activating mutations in K-ras and β-catenin in colonocytes, ctivation of Wnt signaling by accumulation of beta-catenin is a major mechanism in the azoxymethane-induced colon carcinogenesis model.

Specific Mmodeling Methods

Mice: 20-25 g•Male A/J mice&bull
Administration: 5 mg/kg• i.p.• weekly for 6 weeks

Note

Modeling Indicators

Cellular/tissue level: Induced hyperproliferation and microadenoma formation, Increased the cyclin D1 and COX-2 expression.
Molecular changes: Increased the protein expression of pro-TGF-α, pEGFR, pErbB2, pERK, induces K-ras mutations.

Correlated Product(s): /

Opposite Product(s): Gefitinib (HY-50895)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

74.08

Formula

C₂H₆N₂O

CAS No.

25843-45-2

Appearance

Liquid

Color

Colorless to light yellow

SMILES

C/[N+]([O-])=N/C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility

In Vitro:

H₂O : ≥ 200 mg/mL (2699.78 mM)

*"≥" means soluble, but saturation unknown.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (280 KB) SDS (735 KB)

COA (307 KB)

Handling Instructions (2659 KB)

References

[1]. Megaraj V, et al. Role of hepatic and intestinal p450 enzymes in the metabolic activation of the colon carcinogen azoxymethane in mice. Chem Res Toxicol. 2014 Apr 21;27(4):656-62. [Content Brief]

[2]. Whetstone RD, et al. Colon carcinogenesis in wild type and immune compromised mice after treatment with azoxymethane, and azoxymethane with dextran sodium sulfate. Mol Carcinog. 2016 Jul;55(7):1187-95. [Content Brief]

[3]. Fichera A, et al. Epidermal growth factor receptor signaling is required for microadenoma formation in the mouse azoxymethane model of colonic carcinogenesis. Cancer Res. 2007 Jan 15;67(2):827-35. [Content Brief]

Kinase Assay ^[1]	The assay for Azoxymethane-induced in vitro DNA adduct formation is performed. Briefly, microsomes (0.5 to 2.0 mg/mL) are incubated with calf thymus DNA (1 mg/mL) and Azoxymethane (200 μM) in a total volume of 1.0 mL. The assay buffer consists of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl₂, 0.3 M KCl, and 1.5 mM NADPH. Incubations are carried out at 37°C for 60 min in a shaking water bath. An additional 30 nM of NADPH is added after the first 30 min. The reaction is stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA is then extracted for tissue homogenates. Control incubations are performed without NADPH^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Male, 8 to 10 week old, WT-A/J, IECN-A/J, and LCN-A/J mice (8 per group) are treated with either saline or Azoxymethane (7.5 mg/kg BW, s.c.), once weekly for 3 weeks. Mice are sacrificed 6 weeks post-treatment for aberrant crypt foci (ACF) detection. The entire colon is excised. A longitudinal incision is made along the entire length of the colon, which is further cut into two equal-length segments, representing proximal and distal portions of the colon. The segments are dipped in PBS to remove fecal pellets and then kept flat between filter papers in 10% buffered formalin for at least 24 h. Subsequently, the colons are immersed in freshly prepared 0.1% methylene blue for 10 min and rinsed briefly in deionized H₂O to remove excess dye. The colon is mounted carefully on a microscope slide with the mucosal surface side up and viewed under a light microscope. The ACF in the entire mucosal surface of the colon are counted blindly and independently by two investigators and recorded^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Megaraj V, et al. Role of hepatic and intestinal p450 enzymes in the metabolic activation of the colon carcinogen azoxymethane in mice. Chem Res Toxicol. 2014 Apr 21;27(4):656-62. [Content Brief] [2]. Whetstone RD, et al. Colon carcinogenesis in wild type and immune compromised mice after treatment with azoxymethane, and azoxymethane with dextran sodium sulfate. Mol Carcinog. 2016 Jul;55(7):1187-95. [Content Brief] [3]. Fichera A, et al. Epidermal growth factor receptor signaling is required for microadenoma formation in the mouse azoxymethane model of colonic carcinogenesis. Cancer Res. 2007 Jan 15;67(2):827-35. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	13.4989 mL	67.4946 mL	134.9892 mL	337.4730 mL
	5 mM	2.6998 mL	13.4989 mL	26.9978 mL	67.4946 mL
	10 mM	1.3499 mL	6.7495 mL	13.4989 mL	33.7473 mL
	15 mM	0.8999 mL	4.4996 mL	8.9993 mL	22.4982 mL
	20 mM	0.6749 mL	3.3747 mL	6.7495 mL	16.8736 mL
	25 mM	0.5400 mL	2.6998 mL	5.3996 mL	13.4989 mL
	30 mM	0.4500 mL	2.2498 mL	4.4996 mL	11.2491 mL
	40 mM	0.3375 mL	1.6874 mL	3.3747 mL	8.4368 mL
	50 mM	0.2700 mL	1.3499 mL	2.6998 mL	6.7495 mL
	60 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	80 mM	0.1687 mL	0.8437 mL	1.6874 mL	4.2184 mL
	100 mM	0.1350 mL	0.6749 mL	1.3499 mL	3.3747 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Azoxymethane Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Azoxymethane25843-45-2AOMDNA Alkylator/CrosslinkerInhibitorinhibitorinhibit

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Azoxymethane (Synonyms: AOM)

Customer Review

Azoxymethane Related Antibodies

11 Publications Citing Use of MCE Azoxymethane

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Azoxymethane Related Antibodies

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Complete Stock Solution Preparation Table

Azoxymethane Related Classifications

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