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BCIP  (Synonyms: BCIP p-toluidine salt; X-phosphate p-toluidine salt)

Cat. No.: HY-15909 Purity: 99.93%
COA Handling Instructions

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BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

For research use only. We do not sell to patients.

BCIP Chemical Structure

BCIP Chemical Structure

CAS No. : 6578-06-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 44 In-stock
Solution
10 mM * 1 mL in DMSO USD 44 In-stock
Solid
100 mg USD 40 In-stock
500 mg USD 170 In-stock
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Other Forms of BCIP:

BCIP Related Antibodies

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Description

BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

Molecular Weight

433.62

Formula

C15H15BrClN2O4P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

ClC1=C(Br)C=CC2=C1C(OP(O)(O)=O)=CN2.NC3=CC=C(C)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (115.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 25 mg/mL (57.65 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3062 mL 11.5308 mL 23.0617 mL
5 mM 0.4612 mL 2.3062 mL 4.6123 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.93%

COA (258 KB) HNMR (260 KB) CNMR (276 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF / DMSO 1 mM 2.3062 mL 11.5308 mL 23.0617 mL 57.6542 mL
5 mM 0.4612 mL 2.3062 mL 4.6123 mL 11.5308 mL
10 mM 0.2306 mL 1.1531 mL 2.3062 mL 5.7654 mL
15 mM 0.1537 mL 0.7687 mL 1.5374 mL 3.8436 mL
20 mM 0.1153 mL 0.5765 mL 1.1531 mL 2.8827 mL
25 mM 0.0922 mL 0.4612 mL 0.9225 mL 2.3062 mL
30 mM 0.0769 mL 0.3844 mL 0.7687 mL 1.9218 mL
40 mM 0.0577 mL 0.2883 mL 0.5765 mL 1.4414 mL
50 mM 0.0461 mL 0.2306 mL 0.4612 mL 1.1531 mL
DMSO 60 mM 0.0384 mL 0.1922 mL 0.3844 mL 0.9609 mL
80 mM 0.0288 mL 0.1441 mL 0.2883 mL 0.7207 mL
100 mM 0.0231 mL 0.1153 mL 0.2306 mL 0.5765 mL
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BCIP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCIP6578-06-9X-phosphateFluorescent DyeInhibitorinhibitorinhibit

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