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  4. 5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium 

Cat. No.: HY-W045537 Purity: 99.95%
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Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is commonly used as a substrate for phosphatase assays in biochemistry and molecular biology. This compound is converted by phosphatases to a yellow compound that can be easily measured spectrophotometrically or fluorometrically. Furthermore, it has been used as a useful reagent in enzyme-catalyzed reactions and a tool for studying signal transduction pathways.

For research use only. We do not sell to patients.

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium Chemical Structure

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium Chemical Structure

CAS No. : 102185-33-1

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100 mg USD 48 In-stock
250 mg USD 72 In-stock
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1 g   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of 5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium:

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium Related Antibodies

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Description

Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is commonly used as a substrate for phosphatase assays in biochemistry and molecular biology. This compound is converted by phosphatases to a yellow compound that can be easily measured spectrophotometrically or fluorometrically. Furthermore, it has been used as a useful reagent in enzyme-catalyzed reactions and a tool for studying signal transduction pathways.

In Vitro

Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium Related Antibodies
Molecular Weight

370.43

Formula

C8H4BrClNNa2O4P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

ClC1=C(Br)C=CC2=C1C(OP(O[Na])(O[Na])=O)=CN2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 41.67 mg/mL (112.49 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6996 mL 13.4978 mL 26.9957 mL
5 mM 0.5399 mL 2.6996 mL 5.3991 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6996 mL 13.4978 mL 26.9957 mL 67.4891 mL
5 mM 0.5399 mL 2.6996 mL 5.3991 mL 13.4978 mL
10 mM 0.2700 mL 1.3498 mL 2.6996 mL 6.7489 mL
15 mM 0.1800 mL 0.8999 mL 1.7997 mL 4.4993 mL
20 mM 0.1350 mL 0.6749 mL 1.3498 mL 3.3745 mL
25 mM 0.1080 mL 0.5399 mL 1.0798 mL 2.6996 mL
30 mM 0.0900 mL 0.4499 mL 0.8999 mL 2.2496 mL
40 mM 0.0675 mL 0.3374 mL 0.6749 mL 1.6872 mL
50 mM 0.0540 mL 0.2700 mL 0.5399 mL 1.3498 mL
60 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1248 mL
80 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8436 mL
100 mM 0.0270 mL 0.1350 mL 0.2700 mL 0.6749 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Bromo-4-chloro-1H-indol-3-yl phosphate102185-33-1Biochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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