Chlormethine hydrochloride (Synonyms: Mechlorethamine hydrochloride)

Name: Chlormethine hydrochloride
Brand: MedChemExpress
SKU: HY-B1253
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1253 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Chlormethine hydrochloride is a foaming agent that causes necrotic and irritating damage to mucous membranes. Used as an antitumor agent in Hodgkin's disease and lymphoma.

For research use only. We do not sell to patients.

Chlormethine hydrochloride Chemical Structure

CAS No. : 55-86-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
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5 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Chlormethine hydrochloride:

Chlormethine (hydrochloride) (Standard) Get quote

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Purity & Documentation
References
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Description

Chlormethine hydrochloride is a foaming agent that causes necrotic and irritating damage to mucous membranes. Used as an antitumor agent in Hodgkin's disease and lymphoma.

In Vitro

Chlormethine hydrochloride (0.0125-1000 μM, 48 h) inhibits lymphoma cell proliferation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	MDFB, DFB, PBMC, MSL
Concentration:	0.0125, 0.06, 0.3, 1.6, 8, 40, 200, 1000 μM
Incubation Time:	48 h
Result:	Inhibited the proliferation of lymphoma cells and had little effect on natural skin cells.

In Vivo

Chlormethine hydrochloride (0.02%, applied as an ointment, five times a week, ten weeks) has shown activity in restoring follicle activity in C3H/HeJ mice^[3].
Chlormethine hydrochloride (0.04-0.4 ng, intravenous injection, single dose) increases IL-6 levels in the blood of mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C3H/HeJ bald spot disease mice^[3]
Dosage:	0.02%, five times a week, 10 weeks
Administration:	Ointment application
Result:	Was a complete hair cover and the disappearance of CD4+ and CD8+ lymphocytes.

Animal Model:	Inbred CBA, C57BL/6 and Balb/c and colony bred SWISS male mice^[4]
Dosage:	0.04, 0.4ng; single dose
Administration:	Intravenous injection
Result:	Increased blood levels of IL-6 but not IL-1.

Clinical Trial

Molecular Weight

192.51

Formula

C₅H₁₂Cl₃N

CAS No.

55-86-7

Appearance

Solid

Color

White to off-white

SMILES

ClCCN(CCCl)C.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1298.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (519.45 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1945 mL	25.9727 mL	51.9454 mL
5 mM	1.0389 mL	5.1945 mL	10.3891 mL
10 mM	0.5195 mL	2.5973 mL	5.1945 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (519.45 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (746 KB)

COA (243 KB) HNMR (249 KB)

Handling Instructions (2659 KB)

References

[1]. Cao Z, et al. Effect of brentuximab vedotin combined with chlormethine hydrochloride on the treatment of 6 patients with relapsed and refractory Hodgkin lymphoma. Zhonghua Xue Ye Xue Za Zhi. 2015 Jul 14;36(7):575-577. [Content Brief]

[2]. Yuan Yang, et al. Chlormethine Hydrochloride is Not Inferior to Tacrolimus in Treating Steroid-Resistant Nephrotic Syndrome. Kidney Blood Press Res. 2018;43(1):68-79. [Content Brief]

[3]. Liren Tang, et al. Topical mechlorethamine restores autoimmune-arrested follicular activity in mice with an alopecia areata-like disease by targeting infiltrated lymphocytes. J Invest Dermatol. 2003 Mar;120(3):400-6. [Content Brief]

[4]. K Bryniarski, et al. The in vivo and in vitro effects of an alkylating agent, mechlorethamine, on IL-6 production in mice and the role of macrophages. Immunopharmacology. 1996 Sep;34(2-3):73-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.1945 mL	25.9727 mL	51.9454 mL	129.8634 mL
	5 mM	1.0389 mL	5.1945 mL	10.3891 mL	25.9727 mL
	10 mM	0.5195 mL	2.5973 mL	5.1945 mL	12.9863 mL
	15 mM	0.3463 mL	1.7315 mL	3.4630 mL	8.6576 mL
	20 mM	0.2597 mL	1.2986 mL	2.5973 mL	6.4932 mL
	25 mM	0.2078 mL	1.0389 mL	2.0778 mL	5.1945 mL
	30 mM	0.1732 mL	0.8658 mL	1.7315 mL	4.3288 mL
	40 mM	0.1299 mL	0.6493 mL	1.2986 mL	3.2466 mL
	50 mM	0.1039 mL	0.5195 mL	1.0389 mL	2.5973 mL
	60 mM	0.0866 mL	0.4329 mL	0.8658 mL	2.1644 mL
	80 mM	0.0649 mL	0.3247 mL	0.6493 mL	1.6233 mL
	100 mM	0.0519 mL	0.2597 mL	0.5195 mL	1.2986 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Chlormethine hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chlormethine55-86-7MechlorethamineOthersAutoimmunityanti-tumorhair growthInhibitorinhibitorinhibit

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Chlormethine hydrochloride (Synonyms: Mechlorethamine hydrochloride)

Customer Review

Other Forms of Chlormethine hydrochloride:

Chlormethine hydrochloride Related Antibodies

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References

Customer Review

Chlormethine hydrochloride Related Antibodies

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Complete Stock Solution Preparation Table

Chlormethine hydrochloride Related Classifications

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