GF 15

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GF 15 is a potent inhibitor of centrosomal clustering in tumor cells.

For research use only. We do not sell to patients.

GF 15 Chemical Structure

CAS No. : 888042-13-5

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Description

GF 15 is a potent inhibitor of centrosomal clustering in tumor cells.

IC₅₀ & Target

Centrosomal clustering^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCC1937	IC₅₀	> 10 μM Compound: 2, GF-15	Antiproliferative activity against exponentially growing adherent human HCC1937 cells assessed as inhibition of cell proliferation after 72 hrs by luminescence detection based ATPlite assay Antiproliferative activity against exponentially growing adherent human HCC1937 cells assessed as inhibition of cell proliferation after 72 hrs by luminescence detection based ATPlite assay	[PMID: 25872984]
MDA-MB-231	IC₅₀	1.62 μM Compound: 2	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay	[PMID: 27061984]
MDA-MB-231	IC₅₀	1.7 μM Compound: 2	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 28258034]
MDA-MB-231	IC₅₀	2.1 μM Compound: 17	Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22191585]
U2OS	IC₅₀	1.3 μM Compound: 2	Antiproliferative activity against human U2OS cells after 72 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human U2OS cells after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 28258034]
U2OS	IC₅₀	1.45 μM Compound: 2	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay	[PMID: 27061984]

In Vitro

GF 15 leads to multipolar mitosis induction with an EC₅₀ of 900 nM. GF 15 exhibits potent cytotoxicity in a concentration-dependent manner against a broad spectrum of tumor cell types including colon, cervix, glioblastoma, pancreas, leukemia, and myeloma-derived cell lines with IC₅₀s of 1-5 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Treatment of mouse xenograft models of human colon cancer and multiple myeloma with GF 15 results in tumor growth inhibition and significantly prolonged survival^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

428.86

Formula

C₂₃H₂₁ClO₆

CAS No.

888042-13-5

SMILES

O=C1[C@]2(C(OCC3=CC=CC=C3)=CC(C[C@H]2C)=O)OC4=C(Cl)C(OC)=CC(OC)=C14

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Raab MS, et al. GF-15, a novel inhibitor of centrosomal clustering, suppresses tumor cell growth in vitro and in vivo. Cancer Res. 2012 Oct 15;72(20):5374-85. [Content Brief]

Cell Assay ^[1]	Hela cells are treated with GF 15 (0, 0.25, 0.5, 0.75, 1, 1.25 μM) for 24 and/or 48 hours. Cell viability is examined using the MTT assay^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] Beige-nude Xid mice are inoculated s.c. in the right flank with 3×10⁶ OPM2 or HT29 cells. When a tumor is measurable, mice are assigned to a GF 15 treatment group (20, 100 mg/kg) or the control group for 0-20 days. Tumor burden is measured every alternating day using an electronic caliper. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Raab MS, et al. GF-15, a novel inhibitor of centrosomal clustering, suppresses tumor cell growth in vitro and in vivo. Cancer Res. 2012 Oct 15;72(20):5374-85. [Content Brief]

GF 15 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GF 15888042-13-5GF15GF-15OthersInhibitorinhibitorinhibit

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