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  3. Immunology and Inflammatory Disease Models
  4. Encephalomyelitis Models
  5. PLP (139-151)

PLP (139-151) is amino acid residue 139 to 151 of myelin proteolipid protein (PLP) used to induce experimental autoimmune encephalomyelitis (EAE).

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PLP (139-151) Chemical Structure

PLP (139-151) Chemical Structure

CAS No. : 131334-43-5

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Based on 1 publication(s) in Google Scholar

PLP (139-151) Related Antibodies

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Description

PLP (139-151) is amino acid residue 139 to 151 of myelin proteolipid protein (PLP) used to induce experimental autoimmune encephalomyelitis (EAE).

In Vitro

Severe clinical and histological EAE could be induced by adoptive transfer of the peptide-specific T cell line and 3 of 4 T cell clones. The T cell line/clones all responded strongly to PLP (139-151) in in vitro proliferative assays. Line SPL and all of the clones show strong proliferative response to the whole PLP molecule and to PLP (139-151)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PLP (139-151) Related Antibodies
In Vivo

After a single oral administration of Amiodarone to rats, its pharmacokinetic characteristics include: the absorption half-life is approximately 1.83 hours, the elimination half-life ranges from 15 hours (at a dose of 100 mg/kg) to 105 hours (at a dose of 200 mg/kg), and the average oral bioavailability is 39%. Amiodarone is primarily distributed in the lungs, liver, thyroid gland, and adipose tissue, with notably higher drug concentrations in the lungs and adipose tissue compared to other tissues. Following chronic oral administration, Amiodarone shows a significant increase in accumulation in adipose tissue.
Amiodarone can be used for animal modeling to construct pulmonary toxicity models and liver injury models [3][4][5].

Induction of Relapsing-Remitting Experimental Autoimmune Encephalomyelitis (RR-EAE)

Background
Proinflammatory cytokines produced by neuroantigen-specific T cells are considered key factors in the initiation and maintenance of autoimmune encephalomyelitis. PLP (139-151) induces autoimmune encephalomyelitis by activating immune responses mediated by specific CD4 cells, which release proinflammatory cytokines such as IL-17 and IFN-γ. This leads to local inflammation and disruption of neural function, thereby inducing autoimmune encephalomyelitis[5].
Specific Mmodeling Methods
Mice: SJL/J • female and female • 6-week-old
Administration: 100 μg • sc • single dose
Note
1. Subcutaneously inject 100 μg of PLP (139-151) (100 μl, 1 μg/μl) along with 100 μl of complete Freund's adjuvant (HY-153808) to enhance the mouse's immune response to PLP (139-151) .
2. One day after the subcutaneous injection of PLP (139-151) , perform an intraperitoneal injection of 100 μl (2 ng/μl, dissolved in phosphate-buffered saline (PBS) ) of Bordetella pertussis toxin (BPX) to further enhance the mouse's immune response to PLP (139-151) .
3. Monitor the mice daily for changes in body weight and clinical status. Perform neurological evaluations using a clinical score (CS) system with the following scale: 0 for no abnormalities; 0.5 for a drooping tail tip; 1 for a limp tail; 1.5 for hind limb dysfunction; 2 for hind limb weakness; 2.5 for dragging hind limbs; 3 for hind limb paralysis; 3.5 for complete hind limb paralysis with the mouse unable to right itself when placed on its side; and 4 for complete hind limb paralysis and partial front limb paralysis.
4. Isolate mice with early clinical symptoms of encephalomyelitis in separate cages with easier access to food and water. Dehydrated mice should receive an intraperitoneal injection of saline solution at least once a day.
Modeling Indicators
Phenotypic Observations: Mice show weight loss and a significant increase in clinical scores. Histological Changes: Iron deposits are observed in the cerebellum, medulla, and spinal cord of the mice. Molecular Changes: Enhanced uptake of [^18F]DPA-714, a radiotracer for the TSPO protein expressed on the surface of central nervous system microglia and macrophages, is observed in the brainstem and cerebellum of mice via positron emission tomography (PET). Immunohistochemistry reveals significantly increased TSPO and Iba1 (markers of microglia and macrophage activation) immunosignals, indicating activation of microglia and macrophages in regions such as the brainstem and cerebellum.
Correlated Product(s): Complete Freund's adjuvant (CFA) (HY-153808)，Bordetella pertussis toxin
Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1537.79

Formula

C72H104N20O16S
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

His-Cys-Leu-Gly-Lys-Trp-Leu-Gly-His-Pro-Asp-Lys-Phe
Sequence Shortening

HCLGKWLGHPDKF
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (65.03 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6503 mL 3.2514 mL 6.5028 mL
5 mM 0.1301 mL 0.6503 mL 1.3006 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (32.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.6503 mL 3.2514 mL 6.5028 mL 16.2571 mL
5 mM 0.1301 mL 0.6503 mL 1.3006 mL 3.2514 mL
10 mM 0.0650 mL 0.3251 mL 0.6503 mL 1.6257 mL
15 mM 0.0434 mL 0.2168 mL 0.4335 mL 1.0838 mL
20 mM 0.0325 mL 0.1626 mL 0.3251 mL 0.8129 mL
25 mM 0.0260 mL 0.1301 mL 0.2601 mL 0.6503 mL
30 mM 0.0217 mL 0.1084 mL 0.2168 mL 0.5419 mL
40 mM 0.0163 mL 0.0813 mL 0.1626 mL 0.4064 mL
50 mM 0.0130 mL 0.0650 mL 0.1301 mL 0.3251 mL
60 mM 0.0108 mL 0.0542 mL 0.1084 mL 0.2710 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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PLP (139-151) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PLP (139-151)131334-43-5OthersInhibitorinhibitorinhibit

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