Synaptamide (Synonyms: N-Docosahexaenoyl ethanolamine (DHEA); Docosahexaenoyl ethanolamide)

Name: Synaptamide
Brand: MedChemExpress
SKU: HY-100197
Rating: 5.0 (1 reviews)

Cat. No.: HY-100197 Purity: 99.30%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Synaptamide (Dehydroepiandrosteron; DHEA) is an endogenous metabolite and structural analogue of Anandamide. Synaptamide binds to both the cannabinoid-1 and 2 (CB1 and CB2) cannabinoid receptors and has anti-inflammatory properties. Synaptamide is the first small-molecule endogenous ligand of an adhesion G protein-coupled receptor (aGPCR) .

For research use only. We do not sell to patients.

Synaptamide Chemical Structure

CAS No. : 162758-94-3

Size	Price	Stock	Quantity
Solvent
5 mg (67 mM * 200 μL in Ethanol)	USD 72	In-stock	Estimated Time of Arrival: December 31
Solvent
10 mg (67 mM * 400 μL in Ethanol)	USD 114	In-stock	Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Synaptamide

•Ecotoxicol Environ Saf. 2021 Feb 25;214:112005. [Abstract]

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CB1 CB2

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[2]

CB1

633 nM (Ki)

CB2

3843 nM (Ki)

Cellular Effect

Cell Line	Type	Value	Description	References
RBL-2H3	IC₅₀	25 μM Compound: 18; DHEA	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2 Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2	[PMID: 31618024]
RBL-2H3	IC₅₀	25 μM Compound: 18; DHEA	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity incubated for 30 mins prior to CB2 antagonist AM630 addition further incubated for 30 mins followed by Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity incubated for 30 mins prior to CB2 antagonist AM630 addition further incubated for 30 mins followed by	[PMID: 31618024]
RBL-2H3	IC₅₀	7.6 μM Compound: 18; DHEA	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity incubated for 30 mins prior to FAAH inhibitor URB597 addition further incubated for 30 mins followed b Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity incubated for 30 mins prior to FAAH inhibitor URB597 addition further incubated for 30 mins followed b	[PMID: 31618024]
Sf9	IC₅₀	> 50 μM Compound: 18; DHEA	Inhibition of human FAAH expressed in baculovirus infected Sf9 cells using N-(6-methoxypyridin-3-yl)octanamide as substrate measured for 15 mins by fluorescence based assay Inhibition of human FAAH expressed in baculovirus infected Sf9 cells using N-(6-methoxypyridin-3-yl)octanamide as substrate measured for 15 mins by fluorescence based assay	[PMID: 31618024]

In Vitro

Synaptamide (Dehydroepiandrosteron; DHEA) is a weak agonist at both CB1 and CB2 receptors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Synaptamide (10 mg/kg; subcutaneously; daily for 7 days) promotes cognitive recovery in rats subjected to mild traumatic brain injury (mTBI)^[1].
Synaptamide (2-10 mg/kg; adult male C57BL/6J mice) reduced the levels of nociceptive and inflammatory pain-related behaviour over 60 min in the intraplantar formaldehyde assay via both intraperitoneal and local intraplantar administration^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats;260 ± 20 g, age 3 months; mTBI model)^[1]
Dosage:	10 mg/kg
Administration:	Subcutaneously; daily for 7 days
Result:	Reversed the mTBI-induced cognitive deficits.

Molecular Weight

371.56

Formula

C₂₄H₃₇NO₂

CAS No.

162758-94-3

Appearance

Liquid (Density: 0.953±0.06 g/cm³)

Color

Colorless to light yellow

SMILES

CC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CCC(NCCO)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

Ethanol : 25 mg/mL (67.28 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6914 mL	13.4568 mL	26.9136 mL
5 mM	0.5383 mL	2.6914 mL	5.3827 mL
10 mM	0.2691 mL	1.3457 mL	2.6914 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 10 mg/mL (26.91 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 10 mg/mL (26.91 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (100.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Saline
Solubility: ≥ 10 mg/mL (26.91 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.3%

Select Batch:

Data Sheet (266 KB) SDS (393 KB)

COA (237 KB)

Handling Instructions (2659 KB)

References

[1]. Kim HY, et al. Synaptamide, endocannabinoid-like derivative of docosahexaenoic acid with cannabinoid-independent function. Prostaglandins Leukot Essent Fatty Acids. 2013 Jan;88(1):121-5. [Content Brief]

[2]. Rashid MA, et al. N-Docosahexaenoylethanolamine is a potent neurogenic factor for neural stem cell differentiation. J Neurochem. 2013 Jun;125(6):869-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.6914 mL	13.4568 mL	26.9136 mL	67.2839 mL
	5 mM	0.5383 mL	2.6914 mL	5.3827 mL	13.4568 mL
	10 mM	0.2691 mL	1.3457 mL	2.6914 mL	6.7284 mL
	15 mM	0.1794 mL	0.8971 mL	1.7942 mL	4.4856 mL
	20 mM	0.1346 mL	0.6728 mL	1.3457 mL	3.3642 mL
	25 mM	0.1077 mL	0.5383 mL	1.0765 mL	2.6914 mL
	30 mM	0.0897 mL	0.4486 mL	0.8971 mL	2.2428 mL
	40 mM	0.0673 mL	0.3364 mL	0.6728 mL	1.6821 mL
	50 mM	0.0538 mL	0.2691 mL	0.5383 mL	1.3457 mL
	60 mM	0.0449 mL	0.2243 mL	0.4486 mL	1.1214 mL

Synaptamide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Synaptamide162758-94-3N-Docosahexaenoyl ethanolamine (DHEA) Docosahexaenoyl ethanolamideCannabinoid ReceptorendogenousligandinflammatorynociceptiveInhibitorinhibitorinhibit

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Synaptamide (Synonyms: N-Docosahexaenoyl ethanolamine (DHEA); Docosahexaenoyl ethanolamide)

Customer Review

Synaptamide Related Antibodies

1 Publications Citing Use of MCE Synaptamide

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Cannabinoid Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Synaptamide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Synaptamide Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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