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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-124171
    Zinquin ethyl ester
    		≥99.0%
    Zinquin ethyl ester is a fluorescent derivative of Zinquin and is a fluorescent probe of cytosolic zinc ion. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn2+ in the presence of Ca2+ and Mg2+ to produce blue fluorescence.
    Zinquin ethyl ester
  • HY-B0171
    Antipyrine
    		99.98%
    Antipyrine (Phenazone) is an orally active antipyretic and analgesic. Antipyrine can be used as a probe agent for oxidative agent metabolism. Antipyrine also delays gastric emptying (GE) in rats. Antipyrine has been widely used in assessment of hepatic oxidative capacity.
    Antipyrine
  • HY-D1106
    Py-BODIPY-NHS ester
    		98.98%
    BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells.
    Py-BODIPY-NHS ester
  • HY-D0084
    3,3'-Dihexyloxacarbocyanine iodide
    		99.62%
    3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
    3,3'-Dihexyloxacarbocyanine iodide
  • HY-D0048
    5-TAMRA-SE
    		98.25%
    5-TAMRA-SE is an amine-reactive fluorescent agent, and its conjugate produces bright, pH-insensitive orange-red fluorescence with good photostability.
    5-TAMRA-SE
  • HY-D0032
    DAF-2DA
    DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.
    DAF-2DA
  • HY-101888
    Cresyl Violet acetate
    Cresyl Violet acetate is a red fluorescent stain, which can be used to stain neurons.
    Cresyl Violet acetate
  • HY-P2528
    Enhanced Green Fluorescent Protein (EGFP) (200-208)
    		98.91%
    Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. Enhanced Green Fluorescent Protein (EGFP) (200-208) is a common reporter protein and is easy to detect.
    Enhanced Green Fluorescent Protein (EGFP) (200-208)
  • HY-118155
    9-Amino-6-chloro-2-methoxyacridine
    		99.56%
    9-Amino-6-chloro-2-methoxyacridine is a pH sensitive fluorescent probe. 9-Amino-6-chloro-2-methoxyacridine has been frequently used to measure changes in vacuolar pH when a specific substrate crosses the tonoplast through a putative H+/solute antiport system.
    9-Amino-6-chloro-2-methoxyacridine
  • HY-137592
    Nicotinamide 1,N6-ethenoadenine dinucleotide
    		≥99.0%
    Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction.
    Nicotinamide 1,N6-ethenoadenine dinucleotide
  • HY-B1571
    Bromophenol blue
    Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.
    Bromophenol blue
  • HY-D1249
    Calcein (mixture of isomers)
    Calcein mixture of isomers is a calcium-dependent fluorescent molecule. Calcein mixture of isomers can be used to study bone metabolism (in vivo) and to stain depressed areas (in vitro). Calcein mixture of isomers can also be used for fluorometry and EDTA titration of calcium ion.
    Calcein (mixture of isomers)
  • HY-D1817
    Vari Fluor 488-Phalloidin
    Vari Fluor 488-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits green fluorescence when labeled, allowing for fluorescence imaging using FITC channels (Ex/Em=488 nm/513 nm).
    Vari Fluor 488-Phalloidin
  • HY-D1913
    FerroOrange
    		98.2%
    FerroOrange is a fluorescent probe for the detection of unstable divalent iron ions, localized in the endoplasmic reticulum, which emits irreversible orange fluorescence upon encountering divalent iron ions. Excitation/emission wavelength: 542/572 nm.
    FerroOrange
  • HY-W127703
    Octadecyl Rhodamine B chloride
    		99.58%
    Octadecyl Rhodamine B chloride is a cationic amphiphile that can be used for staining cell membranes. Octadecyl Rhodamine B chloride can be used in numerous studies including electronic energy transfer in organized molecular assemblies, membrane structure, and distances of closest approach between protein domains and membranes.
    Octadecyl Rhodamine B chloride
  • HY-125623
    MitoPerOx
    		98.14%
    MitoPerOx is a fluorescent ratio-probe of lipid peroxidation. MitoPerOx can be taken up very rapidly into mitochondria within cells, thereby responding to changes in mitochondrial lipid peroxidation.
    MitoPerOx
  • HY-140947
    TAMRA-Azide-PEG-biotin
    		≥99.0%
    TAMRA-Azide-PEG-biotin is a dye derivative of TAMRA (HY-135640). TAMRA-Azide-PEG-biotin contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    TAMRA-Azide-PEG-biotin
  • HY-118135
    4-Methylumbelliferyl-α-D-galactopyranoside
    		99.76%
    4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal), a substrate for α-galactosidase A (GLA), is a blue pro-fluorogenic substrate. 4-Methylumbelliferyl-α-D-galactopyranoside forms two products, galactose and fluorescent 4MU, upon cleavage by GLA.
    4-Methylumbelliferyl-α-D-galactopyranoside
  • HY-110210
    BODIPY-FL NHS ester
    		99.32%
    BODIPY FL NHS ester (BODIPY FL, SE) is a cell membranes-penatrable amine-reactive fluorescent probe. The maximum excitation/emission wavelength of the BODIPY-FL NHS ester are 502/511 nm, respectively. BODIPY-FL NHS ester has high stability and is insensitive to the polarity, pH and type of solvent, and can maintain stable fluorescence properties under different environmental conditions. BODIPY-FL NHS ester can be used for the synthesis of protease substrates, live cell imaging, protein labeling and immunoassay.
    BODIPY-FL NHS ester
  • HY-15558A
    Hoechst 33258 trihydrochloride
    		98.87%
    Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 trihydrochloride
Cat. No. Product Name / Synonyms Application Reactivity