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  3. MitoPerOx

MitoPerOx is a mitochondrial-targeted, lipid peroxidation-indicating fluorescent probe with BODIPY581/591 fluorophores. The triphenylphosphine cation (TPP+) of MitoPerOx can be selectively enriched in mitochondria (depending on membrane potential) and can be used to detect lipid peroxidation in the inner mitochondrial membrane. Under the action of lipid peroxides, the BODIPY581/591 fluorophores of MitoPerOx shift their emission wavelength from 590 nm (reduced state) to 520 nm (oxidized state), and ratiometric detection can be performed at an excitation wavelength of 488 nm. MitoPerOx can specifically monitor the peroxidation of mitochondrial phospholipids (especially cardiolipin) and is used in the study of oxidative stress-related diseases (such as aging, neurodegenerative diseases, and mitochondrial dysfunction)[1][2].

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MitoPerOx Chemical Structure

MitoPerOx Chemical Structure

CAS No. : 1392820-50-6

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Based on 1 publication(s) in Google Scholar

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Description

MitoPerOx is a mitochondrial-targeted, lipid peroxidation-indicating fluorescent probe with BODIPY581/591 fluorophores. The triphenylphosphine cation (TPP+) of MitoPerOx can be selectively enriched in mitochondria (depending on membrane potential) and can be used to detect lipid peroxidation in the inner mitochondrial membrane. Under the action of lipid peroxides, the BODIPY581/591 fluorophores of MitoPerOx shift their emission wavelength from 590 nm (reduced state) to 520 nm (oxidized state), and ratiometric detection can be performed at an excitation wavelength of 488 nm. MitoPerOx can specifically monitor the peroxidation of mitochondrial phospholipids (especially cardiolipin) and is used in the study of oxidative stress-related diseases (such as aging, neurodegenerative diseases, and mitochondrial dysfunction)[1][2].

In Vitro

MitoPerOx (100 nM; 30 min) detects hydrogen peroxide-induced mitochondrial lipid peroxidation in HEK293 cells by increasing the 520/590 nm fluorescence ratio[2].
MitoPerOx Assay Protocol Cell Culture[2]:
Cells (e.g., HEK293 or fibroblasts) are plated on coverslips in 35 mm dishes and cultured at 37 °C, 5% CO2.
Probe Preparation: Add 100 nM MitoPerOx to phenol red-free medium and incubate at 37 °C for 30 min in the dark.
Washing: Discard medium and wash cells three times with PBS.
Induce oxidative stress: Treat cells with 500 μM H2O2 in Hepes-Tris buffer for 15-30 min. Fluorescence imaging: Confocal microscopy (excitation wavelength 488 nm) was used to collect emission light at 520 nm (oxidized state) and 590 nm (reduced state), and the extent of lipid peroxidation was quantified by calculating the 520/590 nm ratio.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

760.46

Formula

C42H38BBrF2N3OP

CAS No.
Appearance

Solid

Color

Dark purple to black

Emission (Em)

520/590

Excitation (Ex)

490

SMILES

O=C(CCC1=CC=C2[N-]1[B+3]([F-])([N]3=C(C=CC3=C2)/C=C/C=C/C4=CC=CC=C4)[F-])NCC[P+](C5=CC=CC=C5)(C6=CC=CC=C6)C7=CC=CC=C7.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (164.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3150 mL 6.5750 mL 13.1499 mL
5 mM 0.2630 mL 1.3150 mL 2.6300 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3150 mL 6.5750 mL 13.1499 mL 32.8748 mL
5 mM 0.2630 mL 1.3150 mL 2.6300 mL 6.5750 mL
10 mM 0.1315 mL 0.6575 mL 1.3150 mL 3.2875 mL
15 mM 0.0877 mL 0.4383 mL 0.8767 mL 2.1917 mL
20 mM 0.0657 mL 0.3287 mL 0.6575 mL 1.6437 mL
25 mM 0.0526 mL 0.2630 mL 0.5260 mL 1.3150 mL
30 mM 0.0438 mL 0.2192 mL 0.4383 mL 1.0958 mL
40 mM 0.0329 mL 0.1644 mL 0.3287 mL 0.8219 mL
50 mM 0.0263 mL 0.1315 mL 0.2630 mL 0.6575 mL
60 mM 0.0219 mL 0.1096 mL 0.2192 mL 0.5479 mL
80 mM 0.0164 mL 0.0822 mL 0.1644 mL 0.4109 mL
100 mM 0.0131 mL 0.0657 mL 0.1315 mL 0.3287 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MitoPerOx
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HY-125623
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