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  4. Nicotinamide 1,N6-ethenoadenine dinucleotide

Nicotinamide 1,N6-ethenoadenine dinucleotide  (Synonyms: ε-NAD)

Cat. No.: HY-137592 Purity: 99%
COA Handling Instructions

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Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction.

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Nicotinamide 1,N6-ethenoadenine dinucleotide Chemical Structure

Nicotinamide 1,N6-ethenoadenine dinucleotide Chemical Structure

CAS No. : 38806-38-1

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Based on 1 publication(s) in Google Scholar

Nicotinamide 1,N6-ethenoadenine dinucleotide Related Antibodies

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Description

Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction[1].

Molecular Weight

687.45

Formula

C23H27N7O14P2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[C@@H]([C@H]1O)COP(OP(OC[C@H]2O[C@@H]([N+]3=CC=CC(C(N)=O)=C3)[C@@H]([C@@H]2O)O)([O-])=O)(O)=O)N4C5=C(N=C4)C6=NC=CN6C=N5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 250 mg/mL (363.66 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4547 mL 7.2733 mL 14.5465 mL
5 mM 0.2909 mL 1.4547 mL 2.9093 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥99.0%

COA (249 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.4547 mL 7.2733 mL 14.5465 mL 36.3663 mL
5 mM 0.2909 mL 1.4547 mL 2.9093 mL 7.2733 mL
10 mM 0.1455 mL 0.7273 mL 1.4547 mL 3.6366 mL
15 mM 0.0970 mL 0.4849 mL 0.9698 mL 2.4244 mL
20 mM 0.0727 mL 0.3637 mL 0.7273 mL 1.8183 mL
25 mM 0.0582 mL 0.2909 mL 0.5819 mL 1.4547 mL
30 mM 0.0485 mL 0.2424 mL 0.4849 mL 1.2122 mL
40 mM 0.0364 mL 0.1818 mL 0.3637 mL 0.9092 mL
50 mM 0.0291 mL 0.1455 mL 0.2909 mL 0.7273 mL
60 mM 0.0242 mL 0.1212 mL 0.2424 mL 0.6061 mL
80 mM 0.0182 mL 0.0909 mL 0.1818 mL 0.4546 mL
100 mM 0.0145 mL 0.0727 mL 0.1455 mL 0.3637 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Nicotinamide 1,N6-ethenoadenine dinucleotide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nicotinamide 1,N6-ethenoadenine dinucleotide38806-38-1ε-NADFluorescent DyeInhibitorinhibitorinhibit

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