5-TAMRA-SE (Synonyms: 5-TAMRA-NHS ester; 5-Carboxytetramethylrhodamine succinimidyl ester)

Name: 5-TAMRA-SE
Brand: MedChemExpress
SKU: HY-D0048
Rating: 5.0 (2 reviews)

Cat. No.: HY-D0048 Purity: 98.25%: FAQs
Data Sheet SDS COA Handling Instructions

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5-TAMRA-SE is an amine-reactive fluorescent agent, and its conjugate produces bright, pH-insensitive orange-red fluorescence with good photostability.

For research use only. We do not sell to patients.

5-TAMRA-SE Chemical Structure

CAS No. : 150810-68-7

Size	Price	Stock	Quantity
1 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 206	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 5-TAMRA-SE

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5-TAMRA-SE is an amine-reactive fluorescent agent, and its conjugate produces bright, pH-insensitive orange-red fluorescence with good photostability.

In Vitro

Preparing the working solution:^[3]
Dissolve 1 mg of 5-TAMRA-SE in 200 µL of DMSO.
5-TAMRA-SE Labeling for Proteins:^[3]
Proteins was dissolved in solvents and reacted with 5-TAMRA SE, which was dissolved in DMSO. The reaction was carried out on a shaking table at room temperature without light for 4 h, and the reaction was terminated using 0.1% (v/v) TFA. The reactants were separated by preparative RP-HPLC. The molecular weight of the product was identified by ESI-MS.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

527.52

Formula

C₂₉H₂₅N₃O₇

CAS No.

150810-68-7

Appearance

Solid

Color

Pale purple to purple

Emission (Em)

580

Excitation (Ex)

565

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(C(ON5C(CCC5=O)=O)=O)C=C4C([O-])=O)=C2C=C1)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMF : 10 mg/mL (18.96 mM; Need ultrasonic)

DMSO : 5 mg/mL (9.48 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8957 mL	9.4783 mL	18.9566 mL
5 mM	0.3791 mL	1.8957 mL	3.7913 mL
10 mM	0.1896 mL	0.9478 mL	1.8957 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMF 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.25%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (255 KB) HNMR (279 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: 5-TAMRA-SE (5-TAMRA-NHS ester) is a fluorescent agent with red fluorescence, it can be labeled to gene vectors/DNA for observing cellular uptake.
Method: For cell labeling.
1. The gene vectors/DNA NPs are labeled with 5-TAMRA-SE dye.
2. Cells are seeded into the glass plates. After 20 h culture, the cell membrane is labeled with DIO dye and then treated with the same amount of the vectors/DNA NPs in 1 mL fresh RPMI 1640 medium without FBS.
3. The living cells are immediately observed by TIRFM (Olympus, Japan). The fluorescence images are captured every 5 s, and the nanoparticles are analyzed by single-particle tracking (SPT) technique programmed by MATLAB software.

References

[1]. Kierzek R, et al.5′-Amino Pyrene Provides a Sensitive, Nonperturbing Fluorescent Probe of RNA Secondary and Tertiary Structure Formation. J Am Chem Soc (1993) 115:4985-4985

[2]. Schmidt T, et al. Local Stoichiometries Determined by Counting Individual Molecules. Anal Chem (1996) 68:4397-4397

[3]. Huang M, et al. Fluorescently Labeled α-Conotoxin TxID, a New Probe for α3β4 Neuronal Nicotinic Acetylcholine Receptors. Mar Drugs. 2022 Aug 12;20(8):511. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / DMF	1 mM	1.8957 mL	9.4783 mL	18.9566 mL	47.3916 mL
DMSO / DMF	5 mM	0.3791 mL	1.8957 mL	3.7913 mL	9.4783 mL
DMF	10 mM	0.1896 mL	0.9478 mL	1.8957 mL	4.7392 mL
DMF	15 mM	0.1264 mL	0.6319 mL	1.2638 mL	3.1594 mL

5-TAMRA-SE Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-TAMRA-SE150810-68-75-TAMRA-NHS ester 5-Carboxytetramethylrhodamine succinimidyl esterFluorescent DyeInhibitorinhibitorinhibit

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5-TAMRA-SE (Synonyms: 5-TAMRA-NHS ester; 5-Carboxytetramethylrhodamine succinimidyl ester)

Customer Review

5-TAMRA-SE Related Antibodies

2 Publications Citing Use of MCE 5-TAMRA-SE

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

5-TAMRA-SE Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

5-TAMRA-SE Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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