VIP(6-28)(human, rat, porcine, bovine)

Name: VIP(6-28)(human, rat, porcine, bovine)
Brand: MedChemExpress
SKU: HY-P1023
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1023 Purity: 99.69%: FAQs
Data Sheet SDS COA Handling Instructions

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VIP(6-28)(human, rat, porcine, bovine) is an effective antagonist of the actions of exogenous vasoactive intestinal peptide (VIP) on cAMP.

For research use only. We do not sell to patients.

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VIP(6-28)(human, rat, porcine, bovine) Chemical Structure

CAS No. : 69698-54-0

Size	Price	Stock	Quantity
500 μg	USD 168	In-stock	Estimated Time of Arrival: December 31
1 mg	USD 228	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 570	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE VIP(6-28)(human, rat, porcine, bovine)

•Cell Metab. 2022 Nov 11;S1550-4131(22)00490-9. [Abstract]

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Biological Activity
Protocol
Purity & Documentation
References
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Description

VIP(6-28)(human, rat, porcine, bovine) is an effective antagonist of the actions of exogenous vasoactive intestinal peptide (VIP) on cAMP.

IC₅₀ & Target

VIP^[1]

In Vitro

VIP(6-28) is an effective VIP antagonist in the superior cervical ganglion (SCG) , and results obtained using this analog indicate that endogenous VIP can participate in a positive feedback loop in injured sympathetic neurons in which it enhances its own expression. VIP(6-28), when added to short-term cultures of adult SCG at a concentration of 10, 30, or 100 μM, reduces the increase in cAMP levels produced by stimulation with 10 μM VIP by 52, 64, or 81%, respectively. At any of these concentrations tested, VIP(6-28) by itself does not alter cAMP levels. In contrast to its ability to reduce the VIP-stimulated elevation in cAMP levels by 64%, the addition of 30 μM VIP(6-28) to culture medium does not significantly alter cAMP levels measured after stimulation of adult ganglia with either isoproterenol or forskolin (10 μM each). Similar results on the ability of VIP(6-28) to block VIP-stimulated increases in cAMP levels are obtained in neuron-enriched and in non-neuronal cell-enriched dissociated cultures^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2816.28

Formula

C₁₂₆H₂₀₇N₃₇O₃₄S

CAS No.

69698-54-0

Appearance

Solid

Color

White to off-white

Sequence

Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2

Sequence Shortening

FTDNYTRLRKQMAVKKYLNSILN-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (240 KB) RP-HPLC (224 KB) LCMS (89 KB)

Handling Instructions (2659 KB)

References

[1]. Mohney RP, et al. Vasoactive intestinal peptide enhances its own expression in sympathetic neurons after injury. J Neurosci. 1998 Jul 15;18(14):5285-93. [Content Brief]

Kinase Assay
^[1]

Adult rats are killed by decapitation. The SCGs are removed, desheathed, placed in organ culture, and maintained for 24 or 48 hr in F-12 defined medium equilibrated with 95% O2 and 5% CO2. Some ganglia are preincubated for 30 min in medium containing the VIP receptor antagonist VIP(6-28), and then transferred for 24 hr to medium containing both VIP(6-28) and an agonist. In experiments in which cAMP is to be measured, ganglia are removed from animals and preincubated for 30 min in F-12 medium containing 500 μM IBMX to prevent the metabolism of cAMP. Ganglia are then incubated for an additional 30 min in F-12 medium with IBMX and the compound to be studied. When the action of VIP(6-28) is examined, it is added to the medium during the last 5 min of the preincubation and throughout the incubation. Ascorbic acid (0.2 mg/mL) is added to cultures containing isoproterenol to retard oxidation of the catecholamine. No significant differences in peptide levels are detected between ganglia maintained in F-12 alone and those cultured in medium containing ascorbic acid^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Mohney RP, et al. Vasoactive intestinal peptide enhances its own expression in sympathetic neurons after injury. J Neurosci. 1998 Jul 15;18(14):5285-93. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.3551 mL	1.7754 mL	3.5508 mL	8.8770 mL
	5 mM	0.0710 mL	0.3551 mL	0.7102 mL	1.7754 mL
	10 mM	0.0355 mL	0.1775 mL	0.3551 mL	0.8877 mL
	15 mM	0.0237 mL	0.1184 mL	0.2367 mL	0.5918 mL
	20 mM	0.0178 mL	0.0888 mL	0.1775 mL	0.4438 mL
	25 mM	0.0142 mL	0.0710 mL	0.1420 mL	0.3551 mL
	30 mM	0.0118 mL	0.0592 mL	0.1184 mL	0.2959 mL