AcrB-IN-3 

Efflux pump-IN-3 is an AcrB efflux pump inhibitor, with ability to potentiate the effect of antibiotics. Efflux pump-IN-3 inhibits Nile Red (a known substrate of AcrB) efflux. Efflux pump-IN-3 does not disrupts the bacterial outer membrane nor display toxicity in a nematode model^[1].

Efflux pump-IN-3 (compound G10) (8-128 μg/mL) shows outstanding antibacterial synergism with at least one of the antibiotics (ERY, LEV and MIN). Efflux pump-IN-3 show antibacterial synergism with MIN, and reduces the MIC value of ERY by 4-fold at 64 μg/mL^[1].
Efflux pump-IN-3 (50 μM, 100 μM) shows strong inhibitory activity at the lowest concentration of 50 μM, to inhibit Nile Red efflux^[1].
Efflux pump-IN-3 (4-256 μg/mL) does not cause hemolysis of mice red blood cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Efflux pump-IN-3 (compound G10) (128 μg/mL; 72 h) shows no significant and in vivo toxicity against Caenorhabditis elegans^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

519.59

C₃₀H₃₃NO₇

2890177-94-1

O=C(C1=CC(OCCCOC2=CC=CC3=C2C=C(C(N4CCOCC4)=O)C5=C3OC(C)(CC5)C)=CC=C1)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Guo T, et al. Design and synthesis of benzochromene derivatives as AcrB inhibitors for the reversal of bacterial multidrug resistance. Eur J Med Chem. 2023 Mar 5;249:115148.  [Content Brief]