alpha-1,3-N-Acetylgalactosaminyltransferase (Pm1138)  (Synonyms: EC 2.4.1.40(Pm1138))

alpha-1,3-N-Acetylgalactosaminyltransferase (Pm1138) (EC 2.4.1.40(Pm1138)), also namely the ABO system transferase with glycosyl transferase activity^[1].

Product Information
This product is recombinantly expressed in E. coli, with a C-terminal His Tag and a molecular weight of 37 kDa. The storage buffer consists of 20 mM Tris-HCl, pH 7.5, and 10% glycerol. It can be used for the synthesis of blood type A antigen.

Instructions
1. Add substrate according to the reaction system, 5 mM MnCl₂, and appropriately add buffer salt such as Tris-HCl, adjust the pH to 7.5, and add appropriate amount of enzyme (according to the reaction requirements);
2. After mixing, incubate the sample at 37℃ for 2-4h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9067-69-0

Liquid

Colorless to light yellow

[alpha-1,3-N-Acetylgalactosaminyltransferase (Pm1138)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ibrahim-Kosta M, et al. ABO blood group, glycosyltransferase activity and risk of venous thromboembolism. Thromb Res. 2020 Sep;193:31-35.  [Content Brief]