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  4. Ammonium chloride, for cell culture

Ammonium chloride, for cell culture  (Synonyms: Salmiac, for cell culture)

Cat. No.: HY-Y1269C Purity: ≥99.0%
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Handling Instructions Technical Support

Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor.

For research use only. We do not sell to patients.

Ammonium chloride, for cell culture Chemical Structure

Ammonium chloride, for cell culture Chemical Structure

CAS No. : 12125-02-9

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Based on 15 publication(s) in Google Scholar

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Ammonium chloride, for cell culture Related Antibodies

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Description

Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor.

In Vitro

Ammonium chloride (NH4Cl), a lysosomotropic agent that raises intralysosomal pH, reduces the yield of reovirus during infection of mouse L cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ammonium chloride, for cell culture Related Antibodies
In Vivo

Ammonium chloride (0.28 M in drinking water) promotes the survival of myocardial cells in vivo by decreasing contractile dysfunction, cardiac hypertrophy, inflammation, apoptosis and autophagy[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8-9-week-old C57B/L6 mice[1].
Dosage: 0.28 M
Administration: Drinking water (5 mg/kg Doxorubicin once a week for 2 weeks)
Result: Effectively improved Doxorubicin (DOX)-induced cardiomyocyte apoptosis and cardiac dysfunction in mice.
Molecular Weight

53.49

Formula

NH4Cl
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (1869.51 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 18.6951 mL 93.4754 mL 186.9508 mL
5 mM 3.7390 mL 18.6951 mL 37.3902 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥99.0%

SDS (537 KB)

COA (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 18.6951 mL 93.4754 mL 186.9508 mL 467.3771 mL
5 mM 3.7390 mL 18.6951 mL 37.3902 mL 93.4754 mL
10 mM 1.8695 mL 9.3475 mL 18.6951 mL 46.7377 mL
15 mM 1.2463 mL 6.2317 mL 12.4634 mL 31.1585 mL
20 mM 0.9348 mL 4.6738 mL 9.3475 mL 23.3689 mL
25 mM 0.7478 mL 3.7390 mL 7.4780 mL 18.6951 mL
30 mM 0.6232 mL 3.1158 mL 6.2317 mL 15.5792 mL
40 mM 0.4674 mL 2.3369 mL 4.6738 mL 11.6844 mL
50 mM 0.3739 mL 1.8695 mL 3.7390 mL 9.3475 mL
60 mM 0.3116 mL 1.5579 mL 3.1158 mL 7.7896 mL
80 mM 0.2337 mL 1.1684 mL 2.3369 mL 5.8422 mL
100 mM 0.1870 mL 0.9348 mL 1.8695 mL 4.6738 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ammonium chloride, for cell culture Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ammonium chloride, for cell culture12125-02-9Salmiac, for cell cultureBiochemical Assay ReagentsAutophagyheteropolarpHintralysosomalinflammationcardiacdysfunctionLysosomeNH4CLInhibitorinhibitorinhibit

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