B-Raf Antibody

Cat. No.: HY-P80036: FAQs
Data Sheet SDS COA User Guide for Antibodies

B-Raf Antibody is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to B-Raf. It can be used for WB,IHC-P,ICC assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	B-Raf Antibody is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to B-Raf. It can be used for WB,IHC-P,ICC assays with tag free, in the background of Human, Mouse, Rat.
Tag	Free
Molecular Weight	Predicted band size: 84 kDa
Purity	Protein A affinity purified.
Subcellular Location	Nucleus, Cytoplasm, Cell membrane.
Conjugation	Non-conjugated
Modification	Unmodified
Research Field	Cell Biology
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Clonality	Monoclonal
Host	Mouse
Reactivity	Human, Mouse, Rat
SwissProt ID	SwissProt: P15056 Human;SwissProt: P28028 Mouse
Dilution Ratio	WB: 1:500-1:2,000 ;ICC: 1:100-1:500 ;IHC-P: 1:100-1:500 Western blot analysis of extracts from K562(lane 2(20μg), HT-29(lane 3(20μg) and HEK293(lane 4(20μg) ) using B-Raf (HY-P80036) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80036, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application	WB, IHC-P, ICC/IF
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG2b
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human B-Raf.AA range:49-239.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

B-Raf Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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B-Raf Antibody

Biological Activity

Technical Parameters

Properties

Documentation

B-Raf Antibody Related Classifications

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