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  4. MAD2L1 Binding Protein Antibody (YA3063)

MAD2L1 Binding Protein Antibody (YA3063)

Cat. No.: HY-P83318
Data Sheet User Guide for Antibodies Technical Support

MAD2L1 Binding Protein Antibody (YA3063) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3063), targeting MAD2L1 Binding Protein, with a predicted molecular weight of 31 kDa (observed band size: 34 kDa). MAD2L1 Binding Protein Antibody (YA3063) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

Size Price Stock
10 μL USD 70 Ask For Quote & Lead Time
50 μL USD 185 Ask For Quote & Lead Time
100 μL USD 300 Ask For Quote & Lead Time

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  • Biological Activity

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Description

MAD2L1 Binding Protein Antibody (YA3063) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3063), targeting MAD2L1 Binding Protein, with a predicted molecular weight of 31 kDa (observed band size: 34 kDa). MAD2L1 Binding Protein Antibody (YA3063) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.

Background

The protein encoded by The related gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. The protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene ID

9587

Synonyms
MAD2L1BP; CMT2; KIAA0110; MAD2L1-binding protein; Caught by MAD2 protein
Molecular Weight

Predicted band size: 31 kDa; Observed band size: 34 kDa

Purity

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Cell Biology

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

SwissProt ID

Q15013

Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

Application

WB, IHC-P, ICC/IF

Appearance

Liquid

Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

A synthesized peptide derived from human MAD2L1BP

Documentation

MAD2L1 Binding Protein Antibody (YA3063) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

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Product Name:
MAD2L1 Binding Protein Antibody (YA3063)
Cat. No.:
HY-P83318
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