MTNR1A Antibody

MTNR1A Antibody is an unconjugated, approximately 39 kDa, rabbit-derived, anti-MTNR1A polyclonal antibody. MTNR1A Antibody can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in human, mouse, rat, and predicted: chicken, dog, pig, cow, sheep, guinea pig background without labeling.

The related gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. The receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.

4543

Predicted band size: 39 kDa;Observed band size: 60 kDa

affinity purified

Cell membrane; Multi-pass membrane protein.

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

Chicken, Dog, Pig, Cow, Sheep, GuineaPig

P48039

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

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  Western blot analysis of extracts from Jurkat (lane 1) and M-intestine (lane 2) using MTNR1A antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, ICC/IF

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from the midlle of human MTNR1A: 201-280/350