Phospho-AMPK alpha 1 (Ser496) Antibody (YA226)

Cat. No.: HY-P80451: FAQs
Data Sheet SDS COA User Guide for Antibodies

Phospho-AMPK alpha 1 (Ser496) Antibody (YA226) is a non-conjugated and Rabbit origined monoclonal antibody about 64 kDa, targeting to Phospho-AMPK alpha 1 (S496). It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	Phospho-AMPK alpha 1 (Ser496) Antibody (YA226) is a non-conjugated and Rabbit origined monoclonal antibody about 64 kDa, targeting to Phospho-AMPK alpha 1 (S496). It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human.
Tag	Free
Molecular Weight	Predicted band size: 64 kDa
Purity	Protein A affinity purified.
Subcellular Location	Nucleus, Cytoplasm.
Conjugation	Non-conjugated
Modification	Phosphorylated
Research Field	Neuroscience
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human
SwissProt ID	SwissProt: Q13131 Human
Dilution Ratio	WB: 1:500-1:1,000 ;ICC/IF: 1:50-1:200 ;IP: Use at an assay dependent concentration. Western blot analysis of extracts from K562(lane 2(20μg) 、HUVEC (lane 3（20μg) and HEK293(lane 4(20μg)), using Phospho-AMPK alpha 1 (HY-P80451) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80451, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application	WB, ICC/IF, IP
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding Ser496 of Human AMPK alpha 1.AA range:471-520.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (206 KB) User Guide for Antibodies (1077 KB)

Phospho-AMPK alpha 1 (Ser496) Antibody (YA226) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-AMPK alpha 1 (Ser496) Antibody (YA226)

Biological Activity

Technical Parameters

Properties

Documentation

Phospho-AMPK alpha 1 (Ser496) Antibody (YA226) Related Classifications

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