Phospho-PKA RII alpha (Ser99) Antibody

Phospho-PKA RII alpha (Ser99) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 46 kDa, targeting to Phospho-PKA RII alpha (Ser99). It can be used for WB,IHC-P,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat, Pig.

PKA RII alpha: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Free

5576

Predicted band size: 46 kDa

affinity purified

Non-conjugated

Phosphorylated

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat, Pig

P13861

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20

WB, IHC-P, ICC/IF, IP

Liquid

Supplied in phosphate buffered saline (pH 7.4), 150 mM NaCl and 50% glycerol. Preservative: 0.02% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser99 of Human Phospho-PKA R2.AA range:436-485.