1. Antibodies
  2. Primary Antibodies
  3. Monoclonal Antibodies Recombinant Antibodies
  4. PKR Antibody

PKR Antibody

Cat. No.: HY-P80283
COA User Guide for Antibodies

PKR Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 62 kDa, targeting to PKR. It can be used for WB,ICC/IF,IHC-P,IP assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PKR Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 62 kDa, targeting to PKR. It can be used for WB,ICC/IF,IHC-P,IP assays with tag free, in the background of Human.

Tag

Free

Molecular Weight

Predicted band size: 62 kDa

Purity

Protein A affinity purified.

Subcellular Location

Nucleus, Cytoplasm, perinuclear region.

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human

SwissProt ID

SwissProt: P19525 Human

Dilution Ratio

WB: 1:1,000-1:5,000 ;ICC/IF: 1:50-1:100 ;IHC-P: 1:50-1:100 ;IP: Use at an assay dependent concentration.

  • Western blot analysis of extracts from THP-1 (lane 1) and Jurkat (lane 2) and Hela (lane 3) using PKR antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ICC/IF, IHC-P, IP

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human PKR.AA range:500-540.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PKR Antibody
Cat. No.:
HY-P80283
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