RPA32 Antibody (YA679)

RPA32 Antibody (YA679) is a non-conjugated and Mouse origined monoclonal antibody about 32 kDa, targeting to RPA32. It can be used for WB, IHC-P, ICC/IF, IP assays with tag free, in the background of Human, Mouse, Rat.

The related gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. The subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Virus

6118

Predicted band size: 32 kDa

Protein A affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

P15927

WB: 1:500-1:1000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20

WB, IHC-P, ICC/IF, IP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.