Thioredoxin Antibody

Thioredoxin Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 12 kDa, targeting to Thioredoxin. It can be used for WB,IHC-F,IHC-P,ICC/IF,IP assays with tag free, in the background of Human.

Thioredoxin: The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Free

7295

Predicted band size: 12 kDa

affinity purified

Non-conjugated

Unmodified

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P10599

WB: 1:1000-1:10,000 ;IHC: 1:50-1:200 ;IF: 1:100-1:500 ;IP: 1:20

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Thioredoxin/TRX.The exact sequence is proprietary to MCE.