Antimicrobial agent-34 

Antimicrobial agent-34 (compound 4h) is an antibacterial agent (MIC = 1–4 μg/mL), with a clogP value of 9.14. Antimicrobial agent-34 has good plasma stability (HC₅₀ of 131.1 μg/mL) and good membrane selectivity (HC₅₀/MIC is 65.6), with rapid sterilization capability. Antimicrobial agent-34 destroys the integrity of bacterial cell membranes, induces an increase in intracellular reactive oxygen species, and leaks protein and DNA, ultimately leading to bacterial death. Antimicrobial agent-34 demonstrates significant in vivo antibacterial potency in a mouse sepsis model infected with Staphylococcus aureus ATCC43300^[1].

Antimicrobial agent-34 can be stably present in human serum, plasma and blood, with MBCs of 16, 16 and 32 μg/mL, respectively, without losing its antimicrobial effect^[1].
Antimicrobial agent-34 has the strongest antibacterial activity against 6 Gram-positive bacteria, with a wide antibacterial spectrum and a MIC range of 1-4 μg/mL. It also has good antibacterial activity against Klebsiella pneumoniae ATCC10031, Unstable Bacteria ATCC19606, and Escherichia coli ATCC25922, with MICs of 4, 2, and 4 μg/mL, respectively^[1].
Antimicrobial agent-34 (1×-16× MIC) shows rapid antibacterial activity against Staphylococcus aureus, and it is dose-dependent^[1].
Antimicrobial agent-34 (2-16 mg/mL; 0-24 h) is difficult to induce resistance. The resistance test of Staphylococcus aureus ATCC43300 under sub-MIC (1/2 MIC) shows a MIC change of only 2-4 times^[1].
Antimicrobial agent-34 (2-64 mg/mL, 0-20 days) has a strong ability to inhibit biofilm formation and destroy pre-formed biofilm, and it is dose-dependent^[1].
Antimicrobial agent-34 (2-32 mg/mL, 0-20 min) disruptes the transmembrane potential of S. aureus, thereby increasing permeability and effectively increasing the levels of ROS, DNA, and protein in S. aureus^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Antimicrobial agent-34 (5 and 10 mg/kg, i.p., continuously at 12-h intervals for 2 days) has a strong inhibitory effect on Gram-positive bacteria in vivo, significantly reducing the bacterial load in the blood, liver, kidney, spleen and other organs of mice in the sepsis model of mice infected with Staphylococcus aureus ATCC43300. TNF-α and IL-6 in the blood of mice are reduced after treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

512.77

C₃₂H₅₂N₂O₃

CC(C=C1)(CC/C=C(C)\C)OC2=C1C(OCCCNCCCN(CCCC)CCCC)=C(C(C)=O)C=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Fangquan Liu, et al. Design, synthesis and biological evaluation of amphiphilic benzopyran derivatives as potent antibacterial agents against multidrug-resistant bacteria. Eur J Med Chem. 2024 Nov 5:277:116784.  [Content Brief]