2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. BDP 581/591 NHS ester

BDP 581/591 NHS ester is a a borondipyrromethene dye (Ex=585 nm, Em=594 nm) that has relatively long fluorescence lifetime and two photon excitation cross section. BDP 581/591 NHS ester can be used for fluorescence polarization analysis and also reacts with reactive oxygen species (ROS) and alter fluorescence. BDP 581/591 NHS ester is also an NHS ester derivative that can be used to bind primary and secondary amine groups of proteins, peptides and other molecules.

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BDP 581/591 NHS ester Chemical Structure

BDP 581/591 NHS ester Chemical Structure

CAS No. : 654651-21-5

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5 mg USD 300 In-stock
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BDP 581/591 NHS ester Related Antibodies

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Description

BDP 581/591 NHS ester is a a borondipyrromethene dye (Ex=585 nm, Em=594 nm) that has relatively long fluorescence lifetime and two photon excitation cross section. BDP 581/591 NHS ester can be used for fluorescence polarization analysis and also reacts with reactive oxygen species (ROS) and alter fluorescence. BDP 581/591 NHS ester is also an NHS ester derivative that can be used to bind primary and secondary amine groups of proteins, peptides and other molecules.

Molecular Weight

489.28

Formula

C26H22BF2N3O4
CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

591
Excitation (Ex)

581
SMILES

[F-][B+3]1([N-]2C(C=C3[N]1=CC(/C=C/C=C/C4=CC=CC=C4)=C3)=CC(CCC(ON5C(CCC5=O)=O)=O)=C2)[F-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMF : 50 mg/mL (102.19 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0438 mL 10.2191 mL 20.4382 mL
5 mM 0.4088 mL 2.0438 mL 4.0876 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF 1 mM 2.0438 mL 10.2191 mL 20.4382 mL 51.0955 mL
5 mM 0.4088 mL 2.0438 mL 4.0876 mL 10.2191 mL
10 mM 0.2044 mL 1.0219 mL 2.0438 mL 5.1095 mL
15 mM 0.1363 mL 0.6813 mL 1.3625 mL 3.4064 mL
20 mM 0.1022 mL 0.5110 mL 1.0219 mL 2.5548 mL
25 mM 0.0818 mL 0.4088 mL 0.8175 mL 2.0438 mL
30 mM 0.0681 mL 0.3406 mL 0.6813 mL 1.7032 mL
40 mM 0.0511 mL 0.2555 mL 0.5110 mL 1.2774 mL
50 mM 0.0409 mL 0.2044 mL 0.4088 mL 1.0219 mL
60 mM 0.0341 mL 0.1703 mL 0.3406 mL 0.8516 mL
80 mM 0.0255 mL 0.1277 mL 0.2555 mL 0.6387 mL
100 mM 0.0204 mL 0.1022 mL 0.2044 mL 0.5110 mL
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BDP 581/591 NHS ester Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BDP 581/591 NHS ester654651-21-5Reactive Oxygen Speciesborondipyrromethene dyefluorescence polarization analysisROSInhibitorinhibitorinhibit

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