Beta Lactamase Complementation

Definition:

This strategy is based on a protein fragment complementation assay (PCA) of the enzyme TEM-1 beta-lactamase. The approach includes a simple colorimetric in vitro assays using the cephalosporin nitrocefin and assays in intact cells using the fluorescent substrate CCF2/AM. The combination of in vitro colorimetric and in vivo fluorescence assays of beta-lactamase in mammalian cells permits a variety of sensitive and high-throughput large-scale applications.

References:

[1]. Ontobee, Molecular Interactions Controlled Vocabulary, MI_0011, Beta Lactamase Complementation.

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