Bimolecular Fluorescence Complementation

Definition:

The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non- fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy.

References:

[1]. Ontobee, Molecular Interactions Controlled Vocabulary, MI_0809, Bimolecular Fluorescence Complementation.

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