Classical Fluorescence Spectroscopy

Definition:

Classical Fluorescence Spectroscopy is a technique that generates high-resolution images using fluorescence signals. By labeling target proteins with fluorescent dyes or proteins, and exciting them with a specific light source, it enables observation of protein distribution, dynamic changes, and interactions with other molecules in cells. This technique is widely used in cell biology and molecular biology research, particularly for real-time monitoring of protein behavior.

References:

[1]. Phizicky EM, et al. Protein-protein interactions: methods for detection and analysis. Microbiol Rev. 1995 Mar;59(1):94-123.

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