Electrophoretic Mobility Shift Assay

Definition:

This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed.

References:

[1]. Ontobee, Molecular Interactions Controlled Vocabulary, MI_0413, Electrophoretic Mobility Shift Assay.

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