Helicase POLQ-like

Definition:

Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair. Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA). Possesses both DNA unwinding and annealing activities. Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA. Efficiently unwinds substrates containing 3' overhangs or a D-loop. In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing. Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences.

References:

[1]. Roopesh Anand, et al. HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Nature. 2022 Jan;601(7892):268-273. [Content Brief]

[2]. Federica Marini, et al. A human DNA helicase homologous to the DNA cross-link sensitivity protein Mus308. J Biol Chem. 2002 Mar 8;277(10):8716-23. [Content Brief]

[3]. George-Lucian Moldovan, et al. DNA polymerase POLN participates in cross-link repair and homologous recombination. Mol Cell Biol. 2010 Feb;30(4):1088-96. [Content Brief]

[4]. Kei-ichi Takata, et al. Human DNA helicase HELQ participates in DNA interstrand crosslink tolerance with ATR and RAD51 paralogs. Nat Commun. 2013;4:2338. [Content Brief]

[5]. Spencer W Luebben, et al. Helq acts in parallel to Fancc to suppress replication-associated genome instability. Nucleic Acids Res. 2013 Dec;41(22):10283-97. [Content Brief]

[6]. Agnieszka A Tafel, et al. Human HEL308 localizes to damaged replication forks and unwinds lagging strand structures. J Biol Chem. 2011 May 6;286(18):15832-40. [Content Brief]

[7]. Tabitha Jenkins, et al. The HelQ human DNA repair helicase utilizes a PWI-like domain for DNA loading through interaction with RPA, triggering DNA unwinding by the HelQ helicase core. NAR Cancer. 2021 Jan 12;3(1):zcaa043. [Content Brief]

Biomedical Dictionary

The Biomedical Dictionary is a comprehensive and professional collection of biological academic terms and subject datas. All explanations are supported by authoritative books or high impact factor literatures, and you can acquire accurate explanations of the biomedical terms you want to know.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Name
Email *

	Sorry, but the email address you supplied was invalid.