Hematoxylin and eosin (H&E) staining

Definition:

Hematoxylin and eosin (H&E) staining is a common staining method in paraffin sectioning. Hematoxylin dye solution is alkaline, mainly makes the chromatin in the nucleus and the ribosome in the cytoplasm purplish blue; Eosin is an acidic dye that mainly makes the cytoplasm and extracellular matrix components red.

References:

[1]. Grosset AA, et al. Hematoxylin and Eosin Counterstaining Protocol for Immunohistochemistry Interpretation and Diagnosis. Appl Immunohistochem Mol Morphol. 2019 Aug;27(7):558-563. [Content Brief]

Biomedical Dictionary

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