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  3. In-Gel Kinase Assay

In-Gel Kinase Assay

Definition:

In-Gel Kinase Assay is a technique where kinases transfer radioactive phosphate isotopes from nucleotides to substrate proteins for labeling. The labeled substrates are then separated by gel electrophoresis, and the presence of the phosphate label is confirmed via autoradiography. This method is commonly used to study kinase activity, substrate specificity, and their roles in signaling pathways.

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