LexA B52 Complementation

Definition:

LexA B52 Complementation uses the Escherichia coli LexA amino acids 1-202 as the DNA-binding domain and the B42 acidic sequence as the activation domain in a yeast two-hybrid system, analyzing protein-protein interactions through lacZ and LEU2 reporter genes. It is suitable for studies on signal transduction and gene expression regulation.

References:

[1]. Zhong J, et al. A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. Genome Res. 2003 Dec;13(12):2691-9. [Content Brief]

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