Mammalian Protein-Protein Interaction Trap

Definition:

Mammalian Protein-Protein Interaction Trap (MAPPIT) is a protein-protein interaction screening method based on the reconstruction of membrane STAT (transcriptional activator) receptors in mammalian cells. The bait protein is fused with a receptor lacking the STAT recruitment site, while the prey protein is fused with a functional STAT recruitment site. In this configuration, the given bait-prey interaction restores a STAT-dependent response, leading to the expression of a reporter gene. This system can not only display protein interactions but also interactions that are independent of modifications but depend on tyrosine phosphorylation.

References:

[1]. Lemmens I, et al. Heteromeric MAPPIT: a novel strategy to study modification-dependent protein-protein interactions in mammalian cells. Nucleic Acids Res. 2003 Jul 15;31(14):e75. [Content Brief]

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