Mass Spectrometry of Hydrogen/Deuterium Exchange

Definition:

Mass Spectrometry of Hydrogen/Deuterium Exchange is a technique based on mass spectrometry that measures the rate and/or extent of hydrogen/deuterium exchange. By analyzing the dynamics of hydrogen/deuterium exchange in proteins or complexes in solution, this method reveals molecular structural flexibility, stability, and solvent-exposed regions. It is widely applied in protein structure and dynamics studies.

References:

[1]. Andréasson C, et al. Insights into the structural dynamics of the Hsp110-Hsp70 interaction reveal the mechanism for nucleotide exchange activity. Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16519-24. [Content Brief]

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