Protein Kinase Activity Assay

Definition:

Protein Kinase Activity Assay is used to detect the enzymatic reaction where protein kinases catalyze the transfer of phosphate groups from ATP to substrate proteins. This technique is useful for studying kinase functions in signal transduction pathways, inhibitor screening, and characterizing biological activities.

References:

[1]. Witt JJ, et al. Rapid protein kinase assay using phosphocellulose-paper absorption. Anal Biochem. 1975 May 26;66(1):253-8. [Content Brief]

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