Protein Three Hybrid

Definition:

Protein Three Hybrid is based on the classical two-hybrid technique with the addition of a third protein component to detect interactions among the three. In the experiment, the bait protein, prey protein, and the third protein are co-transfected into recombinant yeast. Negative control experiments show that the interaction between bait and prey does not occur unless the third protein is co-transfected. This technique is commonly used to study the assembly and regulatory mechanisms of multi-protein complexes.

References:

[1]. SenGupta DJ, et al. A three-hybrid system to detect RNA-protein interactions in vivo. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8496-501.

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