Reverse Phase Chromatography

Definition:

Reverse Phase Chromatography is used to separate mixtures of molecules with different hydrophobic and hydrophilic properties. The technique uses silica gel matrices with covalently bound n-alkyl chains as the stationary phase. For example, C-18 columns strongly retain hydrophobic compounds, while hydrophilic molecules elute more quickly. This method is widely used in the separation and purification of proteins, peptides, and small molecule drugs.

References:

[1]. Molnar I, et al. Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography[J]. Clinical chemistry, 1976, 22(9): 1497-1502. [Content Brief]

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