Reverse RAS Recruitment System

Definition:

Reverse RAS Recruitment System is used to study interactions between membrane proteins and cytoplasmic proteins, widely applied in signal transduction and cell proliferation research. The bait is a membrane protein (e.g., a receptor or channel protein), and the prey is a fusion protein with a cytoplasmic Ras sequence. When they interact, Ras is recruited to the membrane and activates downstream signaling pathways. The experiment is conducted in yeast strains with temperature-sensitive Ras mutations to prevent spontaneous cell growth.

References:

[1]. Hubsman M, et al. A novel approach for the identification of protein-protein interaction with integral membrane proteins. Nucleic Acids Res. 2001 Feb 15;29(4):E18. [Content Brief]

Biomedical Dictionary

The Biomedical Dictionary is a comprehensive and professional collection of biological academic terms and subject datas. All explanations are supported by authoritative books or high impact factor literatures, and you can acquire accurate explanations of the biomedical terms you want to know.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Name
Email *

	Sorry, but the email address you supplied was invalid.