Solid Phase Assay

Definition:

Solid Phase Assay is used to study interactions when at least one molecule is fixed to a solid surface (such as the walls of microplates or test tubes) while the other reactants freely diffuse in solution. This method is widely used in enzyme-substrate binding, antigen-antibody interactions, and drug screening.

References:

[1]. Kojima-Aikawa K. Solid-phase assay of lectin activity using HRP-conjugated glycoproteins. Methods Mol Biol. 2014;1200:101-6.

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