Split Firefly Luciferase Complementation

Definition:

Split Firefly Luciferase Complementation is used to measure the transfer of radioactive methyl groups (such as S-adenosylmethionine (SAM)) from donor to receptor carboxyl group, widely applied in the study of methylation reactions.

References:

[1]. Xie Q, et al. Firefly Luciferase Complementation-Based Analysis of Dynamic Protein-Protein Interactions Under Diurnal and Circadian Conditions in Arabidopsis. Methods Mol Biol. 2022;2398:205-213. [Content Brief]

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