Three Hybrid Assay

Definition:

The Three Hybrid Assay is used to study the role of third-party molecules in the binding process between bait and prey, particularly significant in transcriptional regulation and protein complex assembly research. This method is based on a complementation experiment, indicating that a third-party molecule is necessary for the binding of a specific bait-prey pair. The molecule fused with the DNA binding domain is the bait, while the molecule fused with the transcriptional activation factor is the prey, revealing complex molecular interaction processes.

References:

[1]. SenGupta DJ, et al. A three-hybrid system to detect RNA-protein interactions in vivo. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8496-501. [Content Brief]

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