Transmission Electron Microscopy

Definition:

Transmission Electron Microscopy is used to form high-resolution images by transmitting an electron beam through a sample, locating target proteins inside cells. It can be used for purified molecules (detecting interactions without staining) or tissues/cells. Tissue sections are incubated with highly specific antibodies conjugated to heavy metals (such as gold) before microscopic analysis, followed by molecular co-localization analysis via electron microscopy.

References:

[1]. Harris JR. Transmission electron microscopy in molecular structural biology: A historical survey. Arch Biochem Biophys. 2015 Sep 1;581:3-18. [Content Brief]

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