Two Hybrid Bait and Prey Pooling Approach

Definition:

The Bait-Prey Pooling Two Hybrid Approach is used to screen for interaction partners between baits and preys, widely applied in constructing protein interaction maps and discovering drug targets. By pairing a single bait with a prey pool or pairing a bait pool with a single prey, this method requires cloning the bait and prey into two separate two-hybrid vectors, followed by grouped screening of the transformant collection. Positive two-hybrid clones are the interacting protein partners, providing a powerful tool for in-depth studies of protein networks.

References:

[1]. Ito T, et al. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4569-74. [Content Brief]

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