Two Hybrid Pooling

Definition:

Two Hybrid Pooling is used for high-throughput studies of interactions between multiple candidate proteins. By combining a single bait protein with a mixed pool of candidate proteins, this method quickly identifies potential interacting proteins.

References:

[1]. Ito T, et al. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4569-74.

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